Plot summary

The film opens at temple of princess Kasumi (Devon Aoki). Hayabusa informs her of the death of her brother. Not wanting to believe, she leaves the temple and becomes an outcast Shinobi. Before leaving she is confronted by Ayane (Natassia Malthe) who states that if Kasumi leaves, she is honor-bound to kill her. Jumping the wall of the temple Kasumi catches a flying Shuriken on which the words "You are invited to DOA" appear.Out at sea, Tina (Jaime Pressly) is on her yacht, arguing with her father on the phone about professional wrestling. A gang of pirates (led by Robin Shou) pull alongside and try to take her boat. Tina defeats them and a DOA Shuriken pierces the side of her boat. In a Hong Kong hotel, Christie (Holly Valance) is being questioned by a number of policemen after a theft of diamonds. Wearing only a towel, she manages to disarm and defeat all of them and drives off on a motorcycle. All the while she is observed by Maximillian Marsh (Matthew Marsden). While driving she receives her own Shuriken.The three girls are among a number of invited fighters, including Tina's father, Bass (Kevin Nash), and Max, on a plane going to DOA island. On the plane they're informed by Helena that in order to reach DOA island they must jump off with parachutes and get to the compound by sundown. Quite coincidentally the three girls land close to one another, and must work together to climb a giant Buddha statue when Kasumi realizes it's getting late. At the compound the fighters go on to meet Dr. Victor Donovan (Eric Roberts), the organizer of this year's DOA, who explains about the $10 million prize money, and some of the history about the tournament. Each fighter is analyzed in a lab to show their strength, and are all injected with nanobots which record their abilities. Weatherby (Steve Howey), who operates all the technical equipment, oversees all of this. After all fighters are issued with identity bracelets, which beep and show the name of the wearer's next opponent in the tournament, the confrontations soon begin, with Tina, Christie, Gen Fu (Fang Liu), Hayabusa (Kane Kosugi), Helena Douglas (Sarah Carter), Bayman (Derek Boyer), Zack (Brian J. White) all progressing to round two.Meanwhile, Kasumi recalls her brother, Hayate (Collin Chou), and how he once saved her from ruthless kidnappers. After saving her, she remembers his face when a DOA Shuriken came to him. Before her first round fight, Donovan is happy to see Kasumi; Donovan explains that Hayate was defeated by Leon the previous year, falling from the top of a cliff with his body never to be found. Kasumi later finds herself also matched against Leon (Silvio Simac); she soon defeats him, realizing that if she can win so easily, Leon could not possibly have beaten Hayate. Hayabusa and Kasumi have both progressed to the semi-final stage of the tournament, and all combatants take a time-out to let off steam. A volleyball tournament takes place down on the beach, with Kasumi and Tina against Helena and Christie. Meanwhile, Hayabusa explores Donovan's compound whilst all eyes are on the game, but is soon captured by the building's booby traps. The volleyball game abruptly ends when the ball is punctured by a throwing star, and Kasumi realizes that Ayane is calling her out.They fight each other in the bamboo treetops, and Kasumi pleads for Ayane's help to find Hayate, pointing out that when he deserted the clan, she never pursued him out of love. Ayane coldly replies that he was different, and that Kasumi should come to terms with his death. Their confrontation ends with the arrival of the other girls, prompting Ayane to flee. With the recreation time over, the tournament resumes with Tina being matched against Zack. The two fight at the forbidden square and Tina defeats him. The result leaves a showdown between Helena and Christie. Before the fight, Max and Christie discuss how a source had said "Helena is the key" to finding the Douglas vault somewhere on the island which contains over $100 million. While fighting on the beach in the rain, Christie notices a tattoo on the back of Helena's neck. Despite being caught off guard by the image, Christie still manages to overpower Helena, leaving her lying unconscious on the wet sand, and progressing to the semi-finals of the tournament.Returning to Max, she recalls and deciphers the cryptic image, showing the location of the vault to be in the Buddha head statue. With a new round beginning, Tina, Christie and Kasumi wonder where Hayabusa has gone, and go looking for him at Donovan's compound. They fall into a trap and are captured. It turns out that Donovan is planning to harvest the nanobots from the four best DOA fighters, creating a technology where the host will be able to predict and adapt to any fighting style and therefore defeat any combatant. The technology had been Weatherby's, who had no idea Donovan would use it against fighters' wills, as well as for profit. Weatherby, who has fallen in love with Helena, reveals to her that her father had objected to the idea and following his objections was killed. After being comforted by Weatherby about her failure against Christie, Helena gains new resolve to stop Donovan. Fighting through all his security forces, the two make it to the compound and prevent Donovan from broadcasting the technology to buyers around the world. Meanwhile, Max finds the Douglas vault and breaks the code, but is interrupted by Bayman, who under Donovan's command knocks out Max and takes the cash.Donovan downloads the DOA program onto special sunglasses. He reveals that he captured Hayate a year ago and much to everyone's surprise, frees him. He challenges Hayate to one fight, in which the technology allows him to effectively predict every move Hayate makes and kicks him through the back wall of the compound. Donovan is about to send the DOA program to buyers across the globe when Weatherby stops him and contacts the CIA. Sometime later, Donovan sets the compound on self-destruct, which Weatherby tried to stop, but the self-destruct sequence was irreversible. Weatherby unlocks the harvest pods to free the four semi-finalists, and Helena also manages to hold off Donovan for a small time. With all the fighters freed, the girls join forces to take on Donovan as he attempts to escape in a four-on-one fight.Also, Ayane is still looking for Kasumi when she sees Hayate. Ayane is happy to see Hayate still alive, and they hug each other. Meanwhile, Hayabusa fends off Bayman with the help of Weatherby and Max, and Hayate is aided by Ayane. After a tough fight, Christie manages to knock off Donovan's glasses, preventing him from using the DOA program. Kasumi and Hayate then finish him off, Hayate knock him down, and Kasumi stabs a needle in the back of his neck, leaving him paralyzed and unable to escape. In the final moments, all the combatants jump off the cliff into the water, whilst Weatherby, Max and Hayabusa take an escape hatch. The compound explodes, killing Donovan.Managing to gain a boat (from the same pirates that attacked Tina earlier on), the group escapes DOA island. Back at Kasumi's palace, the five female fighters join forces against the warrior guards on the steps of the palace. There is a cut in the frame, then the results of the fight is shown - the five of them defeated the entire population of guards in the palace.